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Necrotic cell death 1. In vitro cell death assessment Justification Objectives and Hypothesis 1. Aims and Objectives Effects of MSE and MIT on the growth and survival of human cell lines Introduction Materials and methods 2. Chemicals and reagents 2.
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The crude methanol extract obtained appeared greasy with a dark green colour. The crude methanol extract was re-dissolved in 300 ml chloroform and the mixture was transferred into a separating funnel. Four hundred (400 ml) of distilled water was added to the separating funnel and the mixture was shaken thoroughly then left to stand until two layers were formed. The bottom layer (organic layer) was collected and the upper layer (aqueous layer) was re-extracted with the chloroform again and this step was repeated three times. The collected captain kratom addictive organic layer was filtered through sodium sulphate anhydrous and the organic filtrate was further dried by rotary evaporator. The crude chloroform extract obtained
appeared greasy and very dark green in colour. Analysis of MSE and MIT Dragendorf test was used to confirm the presence of alkaloids in the extract of Mitragyna speciosa Korth.
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