Measurement of ROS with DCFH-DA in SH-SY5Y cells treated with A) H202 how to use dried kratom leaves Kratom Tea Opiate Withdrawal MSE with or without NAC and and B) H202 MIT with or without NAC. The fluorescent readings are normalised to Control group. Kratom Tea Opiate Withdrawal nAC at both 33 and 63 min with Bonferroni post test.
Another flow cytometry analysis was carried out in this chapter this time using double staining with Annexin V kratom indiana legal conjugates-7-AAD to further determine the nature of cell death. Surprisingly this time a similar outcome was observed for both SH-SY5Y and MCL-5 cells and the shifting of the whole populations was evident at much lower concentrations of MSE
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than in the previous PI staining in chapter 2. This phenomenon is obviously due to the treatment effects as the control and lowest concentration of the MSE tested as seen in fig. The hypothesis of plasma membrane opening is supported with this finding. This phenomenon creates disadvantages for this assay as when the whole FACS profile shifts to the right side of the scale the determination of the stages of cell death is difficult to interpret as the cells are no longer located in specific quadrants. This observation is clearly in contrast with the previous cytological examinations which indicated that SH-SY5Y cells treated with high dose of MSE undergo apoptosis rather than necrosis. The right mjb kratom full spectrum tincture shifting phenomenon for MIT treated cells observed in fig.
The level of MSE toxicity for SH-SY5Y and HEK 293 cells was found to be increased 10-fold when metabolic activation system (post mitochondrial rat liver S9 induced with Arochlor 1254) was added to the treatment. This implies that MSE cytotoxicity requires metabolism for its activation and CYP2E1 was thought to be involved in this metabolic activation. However MIT in parallel experiments did not show any enhancement of toxicity in the presence of S9 and was inherently cytotoxic.