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Analysis of MSE and MIT 2. Wound assay 2. Cell viability by Trypan blue exclusion assay 2.

Committee on Mutagenicity of Chemicals in Food kratom zerbals Consumer products and the Environment (COM) play an important role in the assessment of genotoxic chemicals. Best Kratom Online 2013 the genotoxic potential of chemicals requires comprehensive assessment using in vivo and in vitro tests which complement each other in their ability to detect genotoxic agents. In the early stage of the testing ICH has recommended an approach called standard test battery which includes three core tests as below: i) a test for gene mutation in bacteria (the Ames Test). Chemicals giving positive results in the standard battery tests depending on their intended use may need to be tested more extensively whereas negative results will usually provide a sufficient level of assurance of safety (ICH 1997). Based on the ICH recommendation for staged genotoxicity assessment gene mutation in bacteria (the Ames test) was the appropriate first test to be performed; however since the leaves of Mitragyna speciosa Korth have long been used by humans an in vitro test using mammalian cells was thought to be more relevant to perform in the current study. In addition the evaluation of genotoxic potential of MSE and MIT at present is for academic purposes and not a regulatory requirement. The mouse kratom review lymphoma tk gene mutation assay (MLA) is widely used and an accepted test what is pure kratom christopher system for the assessment of mammalian cell gene mutation; it involves assessment of the thymidine kinase (tk) locus using mouse lymphoma L5178Y describe kratom high dupree cells.

Sub-culturing was carried out Best Kratom Online 2013 is kratom safe to take everyday tolono approximately every 48 hrs by dilution with prewarmed medium to the initial density of 2. Cells were harvested upon reaching 80-90% confluence. The media was removed and the cells were washed with D-PBS. One ml Trypsin-EDTA was added spread over the cells surface.

The IC50 following 24 hr treatment of SHSY5Y cells were 91. MSE and MIT respectively. Analyses of MSE by UV-VIS spectroscopy confirmed the presence of MIT-like compound at a level of about 42% of the total extract indicating that the MSE IC50 of 91.

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